Rush Proteomics Research Core
The Rush Proteomics Research Core provides a range of services for the design, execution and analysis of proteomics, the study of the entire array of protein expression by cells, tissues and organisms. Proteomics promotes an understanding of the molecular origins of disease and the treatments that might best address them.
Our services support the identification of proteins, the production and purification of recombinant proteins, and the quantitative analysis of proteins from many biological fluids, including serum, plasma, dried blood spots and saliva. Core personnel will be pleased to assist junior and senior investigators with laboratory procedures in both clinical and translational research. The core also participates in several graduate-level courses to introduce basic proteomics methodology to students and young investigators.
Equipment capabilities
- Bio-Rad Gel apparatus, including the mini-Protean Dodeca cell, which can run 10 gels at the same time for high throughput, Trans-Blot Turbo Transfer System, and Chemidoc XRS digital imaging system with PD Quest and Quantity One software
- ELISA Plate readers and Nanodrop 1000 spectrophotometer
- Waters 2795 HPLC with Waters 996 photodiode array detector, and ESA Coulochem II Electrochemical Detector
Services offered
We offer consultations on experimental design, guidance on choosing the most appropriate platform for specific research objectives and data interpretation, training for self-service activities, performance of procedures as needed and post-experiment analysis. Specific services include the following:
- One- and two-dimensional gel electrophoresis and Western blot
- Computer-aided image capture and analysis
- Protein isolation and high-resolution protein analysis
- Extraction of proteins from formalin-fixed paraffin-embedded (FFPE) archived tissues for profiling of proteins for which no fresh sample is available
- Production and purification of recombinant proteins, using E. coli
- ELISA assay for biomarkers, hormones, proteins and lipids from many biological fluids, including dried blood spots
- Bioinformatics support, using analytic software for gels and Western blots
- Biomarker-discovery algorithms and protein-database searches
Expected turnaround times
Two weeks or less for routine assays
Pricing
View this price sheet. If a service is not listed, please contact us for a free initial consultation.