Rush MicroCT and Histology Core

Micro-computed tomography, or microCT, is an X-ray-based imaging method that “sees” objects in three dimensions. The primary missions of the MicroCT and Histology Core are to provide state-of-the-art 3D ex vivo tissue imaging using microCT, and to provide histology services. Users can contract with the Core for microCT scanning and analysis, or can perform these activities themselves after required training and certification by Core personnel. We do not provide training for histology services.

Equipment capabilities

Both our microCT scanners have software for data acquisition, online/offline reconstruction, 3D visualization and animation, as well as 2D and 3D trabecular bone histomorphometry.  There is a database for tracking specimen-processing and archiving, with the capability to readily download histomorphometric data to spreadsheets or statistical packages.

  • Desktop cone-beam microCT scanner Scanco model 40
    • Nominal best resolution of 6 μm with a real resolution of 9 μm
    • Field of view up to 37 mm and maximum scan length of 70 mm
  • Desktop cone-beam microCT scanner Scanco model 50

    • Nominal best resolution 0.5 μm, with a real resolution of ~1 μm
    • Field of view up to 48 mm; maximum scan length 120 mm
  • Faxitron X-ray cabinet (Model MX-20)
    • Digital images
    • Maximum dimensions of specimen:  8” by 11” (203 mm by 279 mm)
    • Smaller-specimens imaging up to 5x magnification for improved detail
  • Histology-preparation equipment, including saws (Buehler Isomet 5000, Exakt 310 CP band saw), grinder/polisher (Buehler Phoenix 4000), microtome (Leica) and embedding equipment for hard- and soft-tissue histology

Services offered

MicroCT services

  • Ex vivo X-ray micro-computed tomographic scanning of specimens
  • 3D quantification of bone architecture, porosity and density
  • 3D quantification of articular cartilage morphology and proteoglycan content
  • 3D quantification of vascular networks
  • 3D quantification of biomaterials
  • Exportation of images (slices) in TIFF or DICOM format
  • Creation of 3D renderings and animations of scanned objects

Faxitron services

  • Ex vivo X-ray imaging
  • In vivo X-ray imaging

Histology services

  • Hard-tissue (undecalcifed) methylmethacrylate (MMA) processing and embedding

  • Preparation of sections of MMA-embedded specimens, using the cut-and-grind method for histology and/or other specialized imaging techniques, such as backscatter scanning electron microscopy and Fourier transform infrared spectroscopy (FTIR) imaging. These specimens can contain metal implants.
  • Preparation of thin sections of MMA-embedded specimens, using microtome-based methods
  • Histologic staining of MMA-embedded sections (Basic Fuchsin)
  • Decalcification, paraffin processing and embedding
  • Preparation of sections using paraffin-embedded specimens
  • Histologic staining of paraffin-embedded sections with hematoxylin and eosin stain (H&E), Toluidine Blue and others)
  • Immunohistochemistry staining of paraffin-embedded sections

Expected turnaround times

Turnaround times are project-specific and dependent on current core instrument use. Please contact the core for an estimate.

Pricing

Please see this pricing document. Since many projects have custom needs, it is always best to contact core personnel to discuss pricing.

Past clients served / current collaborators

We have performed studies, or trained users, from Rush, the University of Illinois at Chicago, Northwestern University, the University of Chicago, Loyola University-Chicago, the University of Iowa, the University of Arizona, the University of Kentucky, and several companies. These projects have involved straightforward bone phenotyping (e.g., for genetic mouse models), studies of bone regeneration at various sites (long bones, cranial, ectopic), studies of bone-implant contact and peri-implant bone architecture, porosity of synthetic materials, vascular mapping (following contrast-agent perfusion), and articular cartilage imaging (with the use of a contrast agent).

Note: We ask clients to acknowledge use of this core in any published manuscripts.

Contact Us

Director for MicroCT and Hard Tissue Histology Cores

Rick Sumner, PhD
Phone: (312) 942-5501
Email: rick_sumner@rush.edu

Research coordinator for MicroCT Core

Ryan Ross, PhD
Phone: (312) 942-5959
Email: ryan_ross@rush.edu

Research coordinator for histology

Meghan Moran, PhD
Phone: (312) 563-2697
Email: meghan_moran@rush.edu