The purpose of the miRNA Core Lab is to facilitate research at Rush, and beyond, in both the qualitative and quantitative measurement and analysis of microRNA and gene expression in tissues and cell types of interest. The core also has expertise in genomic data analysis, using modern statistical tools.
Equipment capabilities
- Applied Biosystems' advanced quantitative PCR instruments, Thermo Fisher ViiA 7 and 7900HT Fast real-time PCR systems with 96- and 384-well capabilities
- Bio-Rad MJ Research Dyad and PTC 200 PCR systems with multi-block thermals for routine PCR applications
- ChemiDoc XRS with Quantity One software for gel imaging and quantitation
- NanoDrop technology for accurate measurement of quality and quantity for DNA and RNA
Services offered
- DNA/RNA/microRNA preparation from cultured cells or tissues
- DNA and RNA extraction from formalin-fixed, paraffin-embedded (FFPE) archived tissues
- DNA/RNA concentration assays
- PCR and RT-PCR
- Real-time PCR
- DNA/RNA electrophoresis
- Real-time RT-PCR-based gene-expression microarrays
- Experimental design consultation
- Data analysis from genomic experiments
Turnaround times
Expected turnaround time is two weeks or less for routine assays.
Pricing
View price sheet. If a service is not listed, please contact us for a free initial consultation.
Contact Us
Cohn Research Building, Lab 453
1735 W. Harrison
Chicago, IL 60612
Phone: (312 ) 563-2628
Core director
Animesh Barua, PhD
Phone: (312) 942-6666
Email: animesh_barua@rush.edu
Core manager and informatics specialist
Seby Edassery, MS
Email: seby_l_edassery@rush.edu