Our work
The Mamede laboratory studies the interactions between viruses and their hosts. Our current focus of research is on the study of the HIV mechanisms of infection in CD4 T-cells, macrophages, and dendritic cells. We are also interested in studying the intracellular innate immunity mechanisms and cellular responses and adaptations to incoming viral particles. We have special interest in proteins that are regulated by Interferon (Interferon Stimulated Genes) and how their expression promotes or blocks infectivity.
We employ multiple techniques in our research such as long live-cell fluorescence imaging, high and super resolution fixed immunofluorescence, biochemistry, biophysical, and functional assays that report successful and abortive viral infection.
By characterizing the interactions between pathogens and hosts, we hope to develop methods that can be impactful in the advance of scientific tools, knowledge, and in the development of novel therapies and cures to viral infections.
Impact of work
Technology
Funding
- K22 AI140963: Direct live-cell visualization and quantification of interactions between infectious HIV particles in primary target cells
- CHEETAH Center Development Award: Study of the early-steps of the HIV replication cycle of infectious HIV particles (Sub-contract from P50 AI150464)
Our team
- Stephanie Gambut, Research Lab Tech 3
- Charia McKee, PhD Candidate
- Janet Zayas, PhD, Postdoctoral Fellow
- Li Zhang, PhD, Postdoctoral Fellow
Contact us
We welcome inquiries about our research, collaborations and funding. Please contact João Mamede, PhD.