Our equipment

Zeiss Sigma HDVP electron microscope

Features

  • High vacuum and variable pressure modes
  • SEM SmartScan
  • Five detectors-simultaneous usage
    • Secondary electron (SE)
    • Back scatter (BSD)
    • Scanning Transmission Electron Microscopy (STEM)
    • In-lens
    • Variable pressure secondary electron (VPSE)
  • Atlas Imaging Software
  • GEMINI column
  • X-ray spectroscopy

Capabilities

  • 1 nm resolution
  • High-definition imaging
  • Large acceleration voltage range (0.2kV-30kV)
  • High scan speeds (50 ns per pixel)
  • Quantitative elemental mapping
  • Ultra high resolution (32,000 x 32,000 pixels)
  • Automatic site to site mosaic compositions
  • Remote viewing

EM preparation equipment

  • EMS 850 Critical Point Dryer
  • Cressington 108auto Sputter Coater

Janus automated workstation

Features

  • Fully automated interface
  • Modular Dispense Technology
  • Scala Scanning System
  • Varispan
  • VersaTip
  • Accusense
  • WinPREP

Capabilities

  • Hands-off adaptability
  • High throughput with no user intervention
  • High-capacity work station (up to 1536 wells, 96 to 384 tip dispense)
  • Liquid level sensing disposable/washable tips
  • Large range volume (nL to mL)
  • High content screening
  • Variable sample spacing (microplates, deep-well plates, vials, test tubes)
  • “Drag and drop” programming
  • Barcode sample tracking
  • Z-stack optical sectioning
  • Time course imaging
  • 3D imaging
  • FRET/FRAP/ROI Bleach modes
  • Non-tactile Measurement of 3D-Topography
  • Linear unmixing of dyes
  • High resolution (2048 x 2048 pixels)

Opera high content screening system

Features

  • Fully automated interface
  • Cell Explorer Automation Platform
  • Nipkow spinning disc
  • On-board liquid dispensing
  • Environmental Control Unit
  • Acapella High Content Imaging and Analysis Software

Capabilities

  • High quality confocal microplate imaging
  • High throughput/content screening (up to 100,000 images/day)
  • Simultaneous and sequential imaging
  • 24/7 multi-channel data acquisition
  • Online image analysis
  • Environment control (temperature, humidity and CO2)

Zeiss LSM700 confocal microscope

Features

  • ZEN software with Smart Setup
  • Inverted scope
  • Focus linear sensor
  • Variable secondary dichroic (VSD) beamsplitter
  • Four stable, solid-state lasers (405-639 nm)
  • Motorized stage (XYZ)
  • Camera Port
  • 10x, 20x, 40x, 63x Objectives

Capabilities

  • Simple configuration with optimized recording settings
  • Fluorescence measurements
  • Automated positioning, focus and exposure

Axio Observer D1

Features

  • CO2 Module
  • Temperature Module
  • Incubation Chamber
  • Automatic Component Recognition
  • 10x, 10x Plan-NEOFLUAR, 40x Objectives

Capabilities

  • Extended live cell imaging
  • Temperature, CO2 and oxygen concentration stable environment
  • Long term time lapse experiments
  • Automatically detect and configure objectives and filters
  • Differential interference contrast imaging

Histology equipment

  • LYNX II Automated Tissue Processor for Histology and Microscopy (EMS)
  • Fully Automated Rotary Microtome Leica RM2255
  • Ultramicrotome Leica EM UC7
  • Research Cryostat Leica CM3050 S
  • Glass Knifemaker Leica EM KMR3
  • Vibrating Blade Microtome Leica VT1000 S

Odyssey CLx infrared imaging system

Features

  • Quantitation Software
  • Nucleic Acid Gel Imaging Software
  • 25 x 25 cm Scan Area
  • Autoscan

Capabilities

  • In/on-Cell Westerns
  • ELISA
  • Protein Arrays
  • Gel Shift Assays
  • Tissue, organ or small animal imaging
  • Wide range, high sensitivity, 2 color detection
  • RNAi analysis

BD Accuri C6 flow cytometer

Features

  • Two lasers/light scatter detectors
  • Four fixed detectors with optical filters
  • Dynamic Fluorescent Compensation
  • 16 million channels of digital data

Capabilities

  • 10,000 events per second
  • 5 x 106 cells per mL
  • Absolute cell/concentration counts
  • Immunophenotyping
  • Bead-Based Immunoassays
  • Cell Cycle Assays
  • Gene expression/RNAi analysis

EnSpire multimode plate reader

Features

  • Corning Epic Labeler
  • AlphaLISA
  • AlphaScreen
  • Quad-monochromator
  • Temperature Control
  • Bar code reader

Capabilities

  • Label-free technology
  • Ultra-sensitive detection
  • Absorbance scans across any wavelength
  • Cellular receptor/signaling assays
  • Binding assays
  • Wide range of plate formats (6-384 wells)
  • Time-resolved fluorescence
  • Multipattern plate shaker