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Research at Rush > Core Research Resources > Laser Capture Microdissection > Laser Capture Microdissection Core
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Descritpion

Tissues are complex structures containing multiple cell types characterized by phenotypic heterogeneity and differential vulnerability to disease processes. Thus, a key objective for the study of disease pathogenesis is the reliable identification and isolation of vulnerable cell types from biopsied or autopsied tissues for subsequent analysis.

Services

To facilitate this process, laser capture microdissection (LCM) technology employs a high-energy laser source coupled to a microscope to identify and separate histochemically or immunohistochemically defined cells from the remainder of a tissue section, and facilitates transfer of the identified cells to a microfuge tube for downstream genetic analysis and proteomics. For instance, microarray analysis of mRNA extracted from individual or pooled cells via LCM generates a 'genetic signature' of vulnerable cell types that can be compared to non-vulnerable populations. Such comparisons can be made between cells from affected and non-affected subjects (e.g., aged control vs.. Alzheimer's disease), between diseased and non-diseased cell from the same subject (e.g., transformed vs.. neighboring normal cells in cancer), or any number of experimental comparisons. The Rush LCM Core houses the latest manifestation of LCM technology, the ArcturusXT, which combines infrared laser capture with UV laser cutting. This unique advancement allows for the precise excision of defined cells from any sample thickness or type (e.g., anything from 7 um thick paraffin-embedded lung tissue to 40 um thick brain tissue sections). Moreover, the ArcturusXT model has epi-flourescence illumination capacity and a six-position fluorescence filter turret for application-specific filter cubes. Hence, multiple label fluorescence can now be combined with LCM to isolate cell types with more specific phenotypes such as multiple markers for early disease as well as chemical phenotype. The ArcturusXT is also loaded with differential interference contrast capabilities for live cell studies. Finally, it should be noted that in addition to its primary role as an optimal tool for isolating individual cellular RNA for microarray analysis and protein identification, LCM can also be used to isolate DNA for genotyping, analysis of pathogenic protein and DNA modifications. This core technology dovetails well with the Rush Proteomics Core. Likewise, cellular protein can be isolated for mass spectrometric analysis through the Rush Proteomics Core.

Pricing

Rush Cores: Laser Capture Microdissection (LCM) Core pricing:

Description Cost
Instrument Time:  

Training (per person)

Instrument time (per hour)

$50.00

$30.00

Supplies:  
Capsure HS LCM cap cassette (4 caps) $32.00
Capsure Macro LCM cap cassette (4 caps) $20.00
PEN membrane glass slides (50 slides) $225.00


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