Proteome based research is at the cutting edge of today's research endeavor. As new proteins are discovered, signaling networks unraveled and new markers are found for diseases, proteomics-based research holds the promise of solving a host of health care related dilemmas. Proteomics research involves both specific experimental designs and specialized instrumentation. The discipline of proteomics involves intricate protein preparation and separation methods, protein chemistry, mass spectrometry and novel computer-driven processes for the handling and analysis of biological data (bioinformatics).
The primary objective of the Rush Proteomics Core (RPC) is to provide a cost-effective means for investigators to integrate proteomic investigations as part of their major research objectives, regardless of whether they are quantitative or comparative in nature.The Rush Proteomics Core (RPC) provides a comprehensive range of services for the design, execution and analysis of proteomics research. Applications include the identification of proteins and antigens, serum profiling and discovery of novel disease biomarkers, analysis of post-translational modifications, characterization of protein-protein interactions, and identification of molecular pathways involved in health and disease. The RPC supports the research of both junior and senior investigators and assists clinical/translational researchers in conducting laboratory procedures.
1: Consultations on experimental design, post experiment analysis and guidance on the most appropriate experimental platform for specific research objectives and data interpretation in proteomics techniques.
2: Protein extraction, analysis and identification using 1D gel, 2 D Gel and western blots and mass spec instruments.
3: Protein assays from various biological specimens includes serum, culture media and saliva for many markers / serum components. We also provide protein analysis from dried blood spots (DBS).
4: Recombinant Protein Production (Prokaryotic system, E Coli) and 3 stage purification using HPLC to obtain >95% purity.
5: Small molecule assays from biological fluids using HPLC and electro chemical or UV detection.
6: The facility also provides bioinformatics support using analytic software for gels and Western blots (BioRad), biomarker discovery algorithms, protein database searches, and analysis of microarray data from glass microarrays, as well as data analysis for the Affymetrix Gene chip platform. An in-house Linux system is routinely used to analyze and store data. Bioconductor, based on the R statistical program, is used for microarray and proteomic data analysis. Most of the NCBI tools for genomic data analysis are installed, including several Blast programs and sequence retrieval/analysis programs. The Core is also linked to the nearby University of Illinois Research Resources Center (RRC; see below) for additional resources such as a Fourier transform mass spectrometer.
Dr Chunxiang "Kevin" Zhang, MD, PhD – Director
Phone: (312) 942-7837
Seby Edassery –Lab Manager
Phone: (312) 563-2628
453 Cohn Research Bldg.
1735 W. Harrison St.
Chicago, IL 60612
Phone: (312) 563-2628