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Research at Rush > Core Research Resources > MicroCT / Histology Subcore > MicroCT/Histology Subcore
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Objective and Mission

The primary missions of the microCT/histology core are to provide state-of-the-art three-dimensional ex vivo imaging using microCT, a non-destructive x-ray based imaging modality and to provide histology services. The specimens for microCT must have inherent radio opacity (e.g., bone) or can be rendered radio-opaque (e.g., by the use of contrast agents).

MicroCT Services

  • Ex vivo x-ray micro computed tomographic scanning of specimens
  • 3-D quantification of bone architecture and density
  • 3-D quantification of articular cartilage morphology and proteoglycan content
  • Exporting images (slices) in TIFF or DICOM format
  • Creating 3-D renderings and animations of scanned objects


Histology Services

  • Hard tissue (undecalcifed) methylmethacrylate (MMA) processing and embedding
  • Hard tissue (undecalcifed) MMA microtome based thin sections and cut and grind sections, including metal implants
  • Hard tissue (undecalcified) histology
  • Decalcified paraffin processing and embedding
  • Decalcified microtome based thin sections
  • Decalcified histology of bone and soft tissues


Example applications of microCT

  • Bone defect healing
  • Fracture healing
  • Bone-implant interface imaging
  • Cortical and trabecular bone architecture and bone density in transgenic or mutant mice and models of metabolic bone disease
  • Bone graft incorporation
  • Vascular patterns (after appropriate perfusion)
  • Cartilage morphology and proteoglycan content
  • Small animal skeleton imaging
  • Biomaterial scaffold imaging


Resources (Instrumentation and Software)

  • Desktop cone-beam microCT scanner Scanco model 40 
    • Microfocus x-ray source (5-7 μm spot size) which can operate between 55 and 90 kVp
    • Detector with 2048 by 256 elements
    • Nominal best resolution of 6 μm with a real resolution of 9 μm
    • Nominal best resolution of 6 μm with a real resolution of 9 μm
    • Reconstructed image matrix of either 1024 X 1024 or 2048 X 2048
    • Field of view up to 37 mm and maximum scan length of 70 mm
  • Desktop cone-beam microCT scanner Scanco model 50
    • Microfocus x-ray source (5-30 μm spot size) which can operate between 45 and 90 kVp
    • Detector with 3400 by 1200 elements
    • Nominal best resolution of 0.5 μm with a real resolution of 2 μm
    • Reconstructed image matrix of up to 8192 X 8192
    • Field of view up to 48 mm and maximum scan length of 120 mm
  • Open VMS operating system, with complete imaging software for data acquisition, online/offline reconstruction, 3D visualization and animation, as well as 2D and 3D trabecular bone histomorphometry
  • Database for tracking specimen processing and archiving and the histomorphometric data can be readily downloaded to spreadsheets or statistical packages
  • Faxitron x-ray cabinet (Model MX-20)
  • Saws (Buehler Isomet 5000, Exakt 310CP band saw, Shopmaster band saw), grinder/polisher (Buehler Phoenix 5000), microtome (Leica), embedding equipment for hard and soft tissue histology


Clients

  • Researchers across several U.S. universities
  • Researchers in private companies
Contact Information (for pricing not included on price lists, contact Dr. Rick Sumner)
 
Rick Sumner, PhD
Facilitator for microCT and hard tissue histology
E-mail: rick_sumner@rush.edu
(312) 942-5501

Ryan Ross, PhD
Research coordinator for microCT
E-mail: ryan_ross@rush.edu
(312) 563-2697

Meghan Moran, PhD
Research coordinator for histology
E-mail: meghan_moran@rush.edu
(312) 563-2697

Lab location: Ste 506B, Armour Academic Facility, 600 S. Paulina St Chicago Il 60612

Publications: Acknowledgement Statement

As appropriate, please acknowledge in all publications the microCT/Histology Core for providing the use of instrumentation and assistance in conducting your studies.

Publications: Reprints

The microCT/Histology Core would appreciate a copy (hard or electronic) of each research publication that utilized this core facility.

 


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